Application

Research Sub CategoryIntegrins

Anti-Integrin α4 Antibody, cytoplasmic on C-terminus is an antibody against Integrin α4 for use in ELISA, IP, RIA, WB, IH.

Research CategoryCell Structure

ELISA/RIA: 1:1000.

Western blot: 1:1000, non-reduced conditions; blocking should be with 1-3% BSA. Recognizes a 140 kDa human alpha 4 band under non-reducing conditions.

Immunohistochemistry: 1:500

Immunoprecipitation: 5 µL of antibody is sufficient to precipitate alpha4 from 5x106 cells.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: cytoplasmic domain, C-terminus

Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha 4 integrin subunit.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1131

Physical form

Liquid rabbit antiserum with 0.05% sodium azide.

Specificity

Integrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus intracellular.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months upon date of receipt. Avoid repeated freeze/thaw cycles.